Diffusion-ordered spectroscopy (DOSY) seeks to separate the NMR signals of different species according to their diffusion coefficient. A series of spin echo spectra is measured with different pulsed field gradient strengths, and the signal decays are analysed to extract a set of diffusion coefficients with which to synthesise the diffusion domain of a DOSY spectrum.


Varian / Agilent
Pulse sequence:
Doneshot.c. (right click and chose 'save ... as')
Parameter set:
setup_dosy and makedosyparams. (right click and chose 'save ... as')

Pulse sequence Package (Oneshot):
zip file.
Pulse sequence Package (Oneshot45):
zip file.

Pulse Sequence Diagram:

Screen capture of the output from Bruker's nmrsim.mod for Oneshot.


Pelta, MD, Morris, GA, Stchedroff, MJ and Hammond, SJ. 2002. A one-shot sequence for high-resolution diffusion-ordered spectroscopy. Magnetic Resonance in Chemistry 40(13):S147-S152.*

Pelta, MD, Barjat, H, Morris, GA, Davis, AL and Hammond, SJ. 1998. Pulse sequences for high-resolution diffusion-ordered spectroscopy (HR-DOSY). Magnetic Resonance in Chemistry 36(10):706-714.*

Wu, DH and Chen, AD and Johnson, CS. 1995. An improved diffusion-ordered spectroscopy experiment incorporating bipolar-gradient pulses. Journal of Magnetic Resonance, Series A 115(2):260-264.

*Please note that copyright restrictions prevent us from placing the pdf files on our website, however, in most cases we are allowed to distribute these via email so contact us if you need a copy.