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Binding of a porphyrin conjugate of Hoechst 33258 to DNA. II. NMR spectroscopic studies detect multiple binding modes to a 12-mer nonself-complementary duplex DNA

Mon, 03/14/2011 - 12:22 — ralph
TitleBinding of a porphyrin conjugate of Hoechst 33258 to DNA. II. NMR spectroscopic studies detect multiple binding modes to a 12-mer nonself-complementary duplex DNA
Publication TypeJournal Article
Year of Publication2001
AuthorsFrau, S, Bichenkova EV, Morris GA, Douglas KT
JournalNucleosides Nucleotides & Nucleic Acids
Volume20
Pagination145-156
ISBN Number1525-7770
Accession NumberWOS:000167323300010
Abstract

We have probed by H-1 NMR spectroscopy the molecular basis of the interaction between Hoechst 33258 conjugated to a des-metalloporphyrin and a non self-complementary duplex DNA sequence, designed on the known chemical nuclease selectivity of this system. The imino NMR spectra are consistent with two distinct families of structure, that is, PORHOE binding either way along the duplex. 2D spectral, T-2, and linewidth data suggest multiple species within the two conformational families.
URLhttp://apps.isiknowledge.com/UA_GeneralSearch_input.do?product=UA&search_mode=GeneralSearchWOS:000167323300010