Analyzing and correcting spectrometer temperature sensitivity

TitleAnalyzing and correcting spectrometer temperature sensitivity
Publication TypeJournal Article
Year of Publication2001
AuthorsBowyer, PJ, Swanson AG, Morris GA
JournalJournal of Magnetic Resonance
ISBN Number1090-7807
Accession NumberWOS:000171546000006

Stability and reproducibility of the spectrometer are fundamental to the success of many modern NMR experiments. Variation in room temperature is a particularly important source of instability, in part because it can cause coherent artifacts in NMR spectra. Small changes in room temperature lead to corresponding changes in the phases, amplitudes, and frequencies of NMR signals. These can lead in turn to apparently random spectral artifacts such as t(1)-noise in two-dimensional (2D) NMR and to the incomplete cancelation of signals in difference spectra, but also, importantly, to F-1 satellite signals in 2D spectra. These "parallel diagonals" arise from the use of air conditioning, which typically forces room temperature to oscillate within a fixed band. Work to identify, quantify, and suppress sources of temperature sensitivity in a modern 300-MHz spectrometer has led to a greater than 10-fold improvement in the signal-to-artifact ratio. (C) 2001 Academic Press.